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Dernière mise à jour : Mai 2018

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Institut Sophia Agrobiotech

UMR INRA - Univ. Nice Sophia Antipolis - Cnrs

http://www.paca.inra.fr/institut-sophia-agrobiotech_eng/

Master research training - M2

Master research training - M2
Stage NEMATOXpress : This research training concerns a Master degree student for a six month period (from the beginning of january to the end of june 2018) with a strong background in Chemistry and interest in Biology

NEMATOXpress

This research training concerns a Master degree student for a six month period (from the beginning of january to the end of june 2018) with a strong background in Chemistry and interest in Biology and consists in:

  • Modulating DNA expression on nematode pests;
  • Developing a strategy for the transient or stable transformation of the plant-parasitic nematode, genus Meloidogyne.

Root‐knot nematodes (RKNs, Meloidogyne species) are among the most economically devastating plant pathogens in the world. Those with asexual modes of reproduction, such as M. incognita, are particularly damaging pests. These obligate endoparasitic nematodes infect roots of thousands of plant species. The development of new control strategies required a better understanding of the molecular dialogue between RKN and plants. However, the lack of transformation procedure, together with the obligate biotrophy of this species, make it difficult to functionally characterize parasitism genes of interest in the focus of post-genomic analyses. 

Key objectives

  • Explore the possibility of heterogeneous gene to express in juvenile of root knot nematode, genus Meloidogyne.
  • Develop an ‘handles-free’ techniques based on chemically mediated DNA transfection

Key activities

  • Chemical synthesis of a polymerizable monomer capable of achieving a monomolecular of plasmide DNA (pDNA) condensation
  • Physico-chemical characterization of the pDNA complexes: size (DLS), morphology (TEM), charge surface (zeta potential)
  • Evaluation of formulations transfection efficiency (fluorescent confocal microscopy) onto nematodes

Teams involved

ICN (Institut Chimie de Nice) MB team (http://icn.unice.fr/spip.php?article109)

Christophe DI GIORGIO, MCU
Charrat et al. (2015) ChemBioChem 16, 792-804.
Fabio et al. (2005) Biochim Biophys Acta 1724, 203-214.

ISA (Institut Sophia Agrobiotech) IPN team (https://www6.paca.inra.fr/institut-sophiaagrobiotech_eng/Research-teams/IPN)

Bruno Favery, DR & Pierre Abad, DR
Truong et al. (2015) Advances in Botanical Research 73, 294-324.
Abad et al. (2008) Nature Biotechnology, 26, 909-915

Contact persons

  • christophe.di-giorgio@unice.fr
  • bruno.favery@inra.fr