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Dernière mise à jour : Mai 2018

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Institut Sophia Agrobiotech

UMR INRA - Univ. Nice Sophia Antipolis - Cnrs

http://www.paca.inra.fr/institut-sophia-agrobiotech_eng/

Protéome et transcriptome des lamellocytes de Drosophila melogaster: nouvelles fonctions et voies de différentiation.

Vendredi 30 Avril à 11h00 - Sophia Antipolis - INRAE PACA - Visioconférence

Séminaire scientifique
Dans le cadre de la nouvelle animation scientifique ISA, nous aurons le plaisir d'avoir un séminaire de Jean Luc Gatti, équipe ESIM.

Où et comment?

Via zoom, en francais, sur ce lien:

https://inrae-fr.zoom.us/j/94019121749?pwd=U0dTY0FXWlhSRmU3Snd0QnZRWm9TUT09

Meeting ID : 940 1912 1749
Passcode : 6i=RgcY2X5

Résumé :

Chez les larves saines de Drosophila melanogaster, les hémocytes circulants sont les plasmatocytes et les cellules cristallines. Ces hémocytes se forment au cours du développement embryonnaire. Un troisième type d'hémocytes, les lamellocytes, sont rares, mais leur nombre augmente après l'oviposition par une guêpe parasitoïde. Ces lamellocytes proviennent de la mobilisation de cluster sous cutanés et de la différentiation accélérée des glandes lymphatiques. Ces cellules forment des couches successives autour de l'œuf de parasitoïde, conduisant à son encapsulation et à sa mélanisation, et finalement à sa mort. Cependant, le nombre total de lamellocytes par larve reste assez faible même après l'infestation par les parasitoïdes, ce qui rend difficile les études biochimiques directes. Nous avons utilisé une drosophile mutante qui produit de manière constitutive un grand nombre de lamellocytes pour purifier ces cellules et analyser leurs protéines par spectrométrie de masse. Les protéines identifiées indiquent que ces cellules sont hautement adaptées au processus d'encapsulation (reconnaissance, motilité, adhésion, signalisation), mais qu'elles peuvent également avoir plusieurs autres fonctions physiologiques (telles que la sécrétion et l'internalisation des vésicules) régulées par différentes voies de signalisation. Nous avons comparé nos résultats de protéomiques avec des études transcriptomiques antérieures et celles plus récentes utilisant les approches de cellules isolées.
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Title : Proteome and transcriptome of Drosophila melogaster lamellocytes: novel functions and differentiation pathways.

Abstract :

In healthy Drosophila melanogaster larvae, the circulating hemocytes are plasma cells and crystal cells. These hemocytes are formed during embryonic development. A third type of hemocytes, the lamellocytes, are rare, but their number increases after oviposition by a parasitoid wasp. These lamellocytes originate from mobilization of subcutaneous clusters and an accelerated differentiation of lymph glands. These cells form successive layers around the parasitoid egg, leading to its encapsulation and melanization, and finally to its death. However, the total number of lamellocytes per larva remains quite low even after parasitoid infestation, making direct biochemical studies difficult. We used a mutant Drosophila that constitutively produces large numbers of lamellocytes to purify these cells and analyze their proteins by mass spectrometry. The identified proteins indicate that these cells are highly adapted to the encapsulation process (recognition, motility, adhesion, signaling), but that they may also have several other physiological functions (such as secretion and internalization of vesicles) regulated by different signalling pathways. We also compared our proteomic results with earlier transcriptomics studies and more recent studies using single cell transcriptome approaches.