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Abstract

Elected in 2013 "method of the year" by the journal Nature Methods, single cell sequencing now allows profiling of gene expression at the level of individual cells (scRNA-seq). Thanks to recent technical improvements in single cell manipulation and library preparation, scRNA-seq has become a powerful tool for high-throughput and high-resolution transcriptomic analysis. It is now possible to define the transcriptomes of thousands of single cells and to establish the molecular identity card for each cell in a cell population. In this talk, i will cover the recent scRNA-seq developments of the UCAGenomix platform in Sophia-Antipolis including results obtained with our recently acquired 10xGenomics system which allows simultaneous profiling of thousands of cells.