Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free:

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site:, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Google Analytics

Targeted advertising cookies


The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at or by post at:

24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Institut Sophia Agrobiotech Logo Marque Etat - République Française Logo_INRAE_noir Logo Université Côte d'Azur CNRS

Home page

Institut Sophia Agrobiotech

UMR INRA - Univ. Nice Sophia Antipolis - Cnrs

A CRISPR/dCAS9 strategy to modify promoter activity: drought stress tolerance application

Friday, 28 january 2018 - 11:00 - Sophia Antipolis - Inra PACA - Room A010

Scientific seminar
As part of the scientific animation of Institut Sophia Agrobiotech, Joaquin Felipe PAIXAO ROCA, IPN team, will present: "A CRISPR/dCAS9 strategy to modify promoter activity: drought stress tolerance application"


Being sessile organisms, plants have to evolve towards a developmental and physiological flexibility in order to generate adaptation mechanisms against different biotic and abiotic stresses. In an agronomical aspect, these environmental factors impact negatively on plant development, representing an elevated economical cost. Water stress impacts different aspects of plant physiology, and can decrease their growth and productivity. Genetic manipulation of plant genomes can overcome some of these difficulties. The type II CRISPR/Cas system has been adapted in plants to control the genetic modification in a more targeted and precise procedure. The catalytically inactive Cas9 (dCas9) fused to activators/repressors has already been used to regulate transcription in transient transformed tobacco leaves and Arabidopsis plants. To improve the technique, we propose here to use the dCas9 fused to the tripartite activator VPR and two arabidopsis epigenetic modification domains: the Acetyltrasnferase domain from AtHAC1 (AT domain), and the methyltransferase domain from CURLY LEAF (CLF) gene (SET domain).  Our results showed that the dCas9 fused to VPR and AT increased the activity of promoters controlling a reporter gene. The dCas9 fused to the SET domain displayed contrasted results, sometimes activating and sometimes inhibiting the expression of the reporter gene. The strategy was also tested to control the endogene promoter of the transcription factor AtAREB1, known to control key genes in the response to drought stress. The AREB1 expression was increased in plants expressing the dCas9-VPR and dCas9-AT fusions, plants that showed a better tolerance to drought stress. Again, the results with the SET domain variated from one line to another, displaying activation and inhibition of the AREB1 expression, with opposed phenotypes when submitted to water withdrawal. Our data indicates that  targeted epigenetic modifications can be used to modify promoter activity. Here, we improved plant’s response to drought stress, but the approach can be designed to control any promoter.