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Dernière mise à jour : Mai 2018

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Institut Sophia Agrobiotech

UMR INRA - Univ. Nice Sophia Antipolis - Cnrs

http://www.paca.inra.fr/institut-sophia-agrobiotech_eng/

Phytophthora parasitica - Microbiota interactions on host plant surface

Friday, december 18 - 11:00 - Salle A010 - Inra PACA - Sophia Antipolis

Scientific seminar
As part of the scientific seminars of the Institute Sophia Antipolis, Marie Larousse, team IPO, will present her work: "Phytophthora parasitica - Microbiota interactions on host plant surface"

Abstract

The interactions between a pathogen and the host surface resident microbiota are critical to disease outbreak. These interactions shape the distribution, the density and the genetic diversity of inoculum. However for most plant pathogens how each of these interactions acts on disease as a single one or as a member of a functional network remains to be specified. This issue is addressed here through the analysis of two types of interactions involving the polyphagous oomycete P.parasitica : (i) the intraspecific interaction that leads to monospecific biofilm formation by P. parasitica zoospores on plant surface; (ii) the interspecific interactions that occur between P. parasitica biofilm and the prokaryotic microbiota of Solanum lycopersicum rhizosphere. The biology of monospecific biofilm is investigated through the characterization of MUCL, a new oomycete-specific Mucin-like Protein family. Gene profiling, biochemical and immunohistological analyses define the extent of this family and lead to identify three members, PPMUCL1/2/3, as residing in P. parasitica biofilm. The Phytophthora parasitica-Microbiota interaction is explored using first a metagenomic approach. Two microbial metagenomes derived from a soil of a tomato greenhouse is defined and compared after 16S RNA gene sequencing: M1 which corresponds to the sub-rhizospheric microbiota able to colonize the roots of axenic tomato seedlings; M2, the sub-microbiota able to colonize the tomato seedling roots previously coated with P. parasitica monospecific biofilm. A representative collection of microorganisms from M2 were also obtained through in vitro selection on a medium prepared from P. parasitica extract. One thousand and two hundred isolates were screened for impact on P. parasitica growth; 1.2% and 9.8% of isolates had a helper or inhibitor growth effect mediated by secreted metabolites. From these isolates, GFP expressing strains were generated and found to be associated with P. parasitica biofilm in which they may modulate oomycete gene expression for mucin-like proteins or effectors that promote successful infection. Taken together these results indicate that at the early step of plant infection a sub-microbiota is physically associated with the oomycete and may interfere with its growth and virulence.