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Dernière mise à jour : Mai 2018

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Institut Sophia Agrobiotech

UMR INRA - Univ. Nice Sophia Antipolis - Cnrs

http://www.paca.inra.fr/institut-sophia-agrobiotech_eng/

Development of RNAi in a Drosophila endoparasitoid wasp and demonstration of its efficiency in impairing venom protein production (ESIM)

Endoparasitoid wasps are essential regulators of insect pests in ecosystems as well as important biological control auxiliaries. Traits important for parasitism success, such as the injection of venom proteins at oviposition, have thus been mainly studied. However, identification of the key genes involved among the large number of genes identified was still prevented by the lack of functional approaches. Here, we report the development of RNA interference (RNAi) in Leptopilina boulardi, a parasitoid that performs its development inside the Drosophila host. Having set up conditions for in vitro development of parasitoid late larval stages or pupae, we targeted the cinnabar gene by microinjecting double-stranded RNA, leading to its silencing and production of red-eyed individuals. We then demonstrated that expression of the gene encoding LbGAP, a virulence factor found in a high amount in L. boulardi venom, could be specifically and almost completely silenced. This is the first report of the efficient silencing of venom protein-encoding genes in parasitoid wasps. Overall, RNAi opens the way for a large-scale functional analysis of parasitoid venom factors as well as other traits involved in parasitism success and more largely in the biology of these ecologically important organisms.

development of RNAi in a Drosophila endoparasitoid wasp

Graphical abstract illustrating the development of RNAi in a Drosophila endoparasitoid wasp.

  • Colinet, D., Kremmer, L., Lemauf, S., Rebuf, C., Gatti, J.-L., and Poirié, M. (2014). J. Insect Physiol. 63, 56–61.