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INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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LEBARON Caroline

TRNO Inra - TECHNICIENNE

Lebaron
Equipe REDD

Functions - Thematic Research

Research technician in Resistance to virus team

- Study of natural and induced allele of multigene family (eIF4E factors) to understand resistance mechanism of virus. 

- Natural allele characterization on Tomato.

- Implementation technical on Molecular Biology and on pathology for candidate resistance gene test.

- Implementation of biotechnology approaches to generate new resistance systems (TILLING, CRISPR/CAS9).

 

Career and Cursus:

Career:

Since 2010: Research technician at INRA Avignon, Resistance to virus team.

2007-2010: Engineer assistant at CNRS UPS 3044 « Baculovirus and therapy » St Christol Lez Ales. Cloning and development of human antibodies by Baculovirus system.

Cursus:

2006-2007 : Professional Licence of Molecular Biology (IUT des Pays de l’Adour)

2004-2006 : Diplôme Universitaire Technologique Génie Biologique option ABB (IUT du Maine)

 

Skills :

Molecular Biology

-          Genotyping (dCAPS and KASPar)

-          Nucleic acid extraction (ARN, ADN)

-          Cloning (Bacteria, Gateway, CRISPR)

-          Yeast: protein-protein interaction by yeast two-hybrid, complementation to test functionality gene.

Cellular Biology

-          Transformation on tomato by Agrobacteria

Plant and Phytopathology

-          Crossing on Tomato and Pepper.

-          Maintenance of viral strain

-          Resistance test: virus inoculation on tomato, pepper and Arabidopsis thaliana, and virus detection by immunoenzymatics method (ELISA).

 

Publications/Posters

2016

LebaronC, Rosado A, Sauvage C, Gauffier C, German-Retana S, Moury B, Gallois JL. A new eIF4E1 allele characterized by RNAseq data mining is associated with resistance to potato virus Y in tomato albeit with a low durability. J Gen Virol. 2016 Nov;97(11):3063-3072. doi: 10.1099/jgv.0.000609.

Gauffier C., Lebaron C., Constant C., Moquet F., Bonnet G., Moretti A.,  Caranta C., Gallois JL. (2016). A TILLING approach to generate broad-spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy. Plant J. 2016 Mar;85(6):717-29. doi: 10.1111/tpj.13136

2015

Jacobin-Valat MJ, Laroche-Traineau J, Larivière M, Mornet S, Sanchez S, Biran M, Lebaron C, Boudon J, Lacomme S, Cérutti M, Clofent-Sanchez G. (2015). Nanoparticles functionalised with an anti-platelet human antibody for in vivo detection of atherosclerotic plaque by magnetic resonance imaging. Nanomedicine. 2015 May;11(4):927-37. doi: 10.1016/j.nano.2014.12.006. Epub 2015 Feb 12.

Lebaron C, Sauvage C, Gauffier C, Moury B, Gallois JL (2015). A new eIF4E allele characterized by RNAseq in tomato is associated with resistance to potyviruses. 15ème rencontre de virologie végétale, Aussois, France, Poster.

Email : caroline.lebaron@inrae.fr